Background Intracranial pressure is measured continuously, and nursing behaviors have been associated with variations in the measurements. Methods A prospective pilot observational study was done to develop a comprehensive list of nursing behaviors that affect patients’ intracranial pressure. Data on nurses were obtained by self-reports and video recording. Patient-level data were collected via chart abstraction, video recording, and patients’ monitors. Results Data on 9 patients and 32 nurses were analyzed. A total of 6244 minutes of data were video recorded. Intracranial pressure was changed because of a nursing intervention during 3394 observations. Compared with baseline levels, intracranial pressure was significantly higher if a nursing intervention was performed (odds ratio, 1.96; 95% CI, 1.71–2.24; P < .001). Conclusion Studying nursing behaviors is feasible. Synchronizing and analyzing mutually exclusive and exhaustive behaviors indicated that nursing behaviors have an effect on patients’ intracranial pressure.